胶体金免疫层析试纸条快速定量检测Asia 1型口蹄疫病毒含量方法的建立

doi:10.11843/j.issn.0366-6964.2014.08.014

Abstract

Abstract:

The study was conducted to develop a rapid and quantitative colloid gold immuno-chromatography assay of Asia 1 type Foot-and-Mouth disease (FMD) virus.Aiming at detecting FMD virus of Asia 1 type quantitatively，the colloid gold-based immuno-chromatographic test strip was prepared and the standard curve was constructed.Testing the laboratory samples and field samples，results showed that this assay had good sensitivity and specificity.This method could accomplish qualitative and semi-quantitative detection in 15 minutes; the sensitivity was up to 0.78 μg•mL^－1 with a linear ranging from 0.78 to 7.84 μg•mL^－1.The coefficient of variation was 0.2% to 5%，and the recovery reported to be 91% to 102%.Good reproducibility and accuracy of this method was demonstrated.The colloidal gold immuno-chromatographic assay is rapid，specific，sensitive，accurate，worthy to recommend its clinical use.

CLC Number:

S852.659.6

ZHU Yu-chan，LIN Mi，SUN Yan-yan，SUN Ji-guo，JIANG Tao. Development of a Rapid and Quantitative Detection of Asia 1 Type Foot-and-Mouth Disease Virus Using Colloid Gold Immuno-chromatography Assay[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1302-1308.

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Development of a Rapid and Quantitative Detection of Asia 1 Type Foot-and-Mouth Disease Virus Using Colloid Gold Immuno-chromatography Assay

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